When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Temperature variation ± 0. Plasma Tau was determined by high-performance liquid chromatography (Shimadzu ®, model LC 10AD) using a Shimadzu model RF 535 fluorescence detector. 95. 7°C and the bottom to 36. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Figure 1. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 1. When a feature or capability applies to only certain readers, this exception is noted. spr. The Multiskan Sky microplate reader is an absorbance plate reader. The SpectraMax 190 reader can be integrated with Molecular . The acceptance. reader in the visible range between 405–750 nm with NIST and NMI. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. Levels of immunoreactivity were normalized to. com 13. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. 6-384 (96- and 384-well with pipetting) 6-384. Five preferred detection modes. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. RAW 264. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. In the M2, M5 and Plus. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. Introduction. Molecular Devices. FlexStation™ I. Examples: “Press carefully to engage,” “ Do not. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. Read More. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax M5 reader settings are shown. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. SpectraMax M5e Multi-Mode Microplate Reader. Plate chamber design of the SpectraMax category 1 microplate readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideFluorescence Microplate Reader Comparison Chart. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. M3-M5e_manual. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax® M5. When a feature or capability applies to only certain readers, this exception is noted. 96-1536. The CDWIA was performed on each. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Recertification is recommended every two years. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Softmax Pro GxP Software 6. FlexStation™ I. Molecular Devices. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. View the Molecular Devices website. Fluorescence Microplate Readers. 2. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. The dual monochromator optics allow the widest. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Thank you!. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. M3-M5e_manual. The raw data from each individual experiment were normalized to vehicle-treated HK-2. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. . Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Microbiota DNA was. The assay window spanned four orders of magnitude. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. 00 USD. , Sunny-vale, CA). AquaMax® 2000. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. #M5) SpectraMax® L Microplate Reader (cat. Molecular Devices Spectramax M5 Microplate Reader. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Our absorbance plate readers feature our PathCheck Sensor technology and. 67. 05% Tween 20 using a Nunc ELISA plate washer. Microplate Readers. 5 12. M3 M4 M5 M5e LM1 v<nnn>. Catalog Number: (89212-396) Supplier: Molecular Devices. SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. Physical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Maximum signal with lowest background. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. 96-384. AquaMax® 4000. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Step 1. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). SoftMax Pro. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. SpectraMax Glo Steady-Luc Reporter Assay Kit. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Use similar settings for other Molecular Devices readers with fluorescence detection mode. 05 3. 0 OD for earlier models. 5°C at 37°C. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. ATP standard curves read on SpectraMax microplate readers. 1. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Italic and boldface type are used for emphasis. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. Fill a clean plate with water. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. 1. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Incubation. 2. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. No filter change with dual monochromator optics. Most papers they cultured bacteria to OD600>0. Designed to provide the simplicity, flexibility and power required for advanced data analysis. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. 00 USD. 0 out of 5 $ 17,999 $ 16,995. com. After being incubated in the dark at 60 ℃ overnight, the supernatants were centrifuged and optical densities were measured at 750, 665, 652 and 470 nm using the SpectraMax M5 microplate reader (Molecular Devices,. Learn how you can utilize microplate readers to drive landmark discoveries. M3-M5e_manual. Maximum signal with lowest background. When a. Optical absorbance. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The SpectraMax M2 and M2e are versatile and reliable. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Five preferred modes for diverse bioresearch applications. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The following document is intended to demonstrate setup of this instrument. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. The advantage of using labeled probes is detection specificity. Microplate Readers include the M3, M4, M5, and M5e models. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). E Models Only) 69. Published Dec 16, 2020 | Updated Dec 16, 2020. Page 28 Chapter 5: Read Modes and Read Types 1. SpectraMax® Aquamax. Molecular Devices Spectramax M5 Microplate Reader. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. While they play an important role in generating data from a wide range of. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. Turn on the microplate reader. The SoftMax® Touch. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. As. ) by nonlinear regression using. SpectraMax® iD5. Bookings are free, but you will need to use iLab to book. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. The cells were harvested on ice and. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. 00. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0 2. begin reading microplates with seven mouse clicks. SpectraMax® M4. 2. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 1. Bookings are free, but you will need to use iLab to book. This. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 1. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 26 1. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Has anyone had problems with the built-in computer of the Spectramax ID3. Add to Cart. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The PBS solution (10. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 72 MB. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. 2. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Expanded wavelength coverage. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. TNF standard curve. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. Choose cables that meet the requirement described in this appendix. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. 7°C (gradient heating design). FlexStation® 3 Multi-Mode Microplate Reader. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Choose from a. When a feature or capability applies to only certain readers, this exception is noted. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Five. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Microplate Reader Documentation Version No. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. p. Figure 1. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. At the end of this period, cells were exposed to FA (1 mM) for 1 h. The dual monochromator optics allow the. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. 95. In . Cuvette port functionality can also be controlled using SoftMax Pro Software. For a higher . We would like to show you a description here but the site won’t allow us. The spectrophotometric. Microplate formats. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. These lab workhorses are used in drug discovery, research, bioassay validation, and. Pick the best speed, sensitivity, and price for your needs. Microplate Readers include the M3, M4, M5, and M5e models. The assay applies fluorescence polarization to quantify Fc-containing IgG. 63 1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The drawing shows a standard microplate of 15 mm height covered with a lid. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. both cuvette-based and microplate reader applications The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 2. Add to Cart. Figure 3. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 6 for further experiment, so I'm confused which i would choose for OD value determination. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. FlexStation™ II. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. However, when a specific probe is not available, or it causes off-target effects on the. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The cuvette must be in place when you read the plate. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 1. The dual monochromator optics allow. Figure 4: Transcreener ADP. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. 0 out of 5 $ 24,695. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax Spectrofluorometers. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Microplate reader settings. When wells are assigned to template groups, data will populate group tables where analysis canThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The K d was calculated with GraphPad Prism software (GraphPad Software Inc. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. We would like to show you a description here but the site won’t allow us. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Microplate Reader Shelf. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Five preferred detection modes. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). Category 2 and 3 microplate readers use gradient heating as well. Analyst, M5, Flex 3, and SpectraMax L readers work differently. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Fluorescence Microplate Reader Comparison Chart. Lower limits of detection for ATP determined using the ATPlite . SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. SpectraMax iD5 Multi-Mode Microplate Reader. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Ltd. 49 2. flexibility of monochromator-based systems. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Wide range of applications The VersaMax reader covers. Most papers they cultured bacteria to OD600>0. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). With optimized reagents, option to operate the readers. Qualify the luminescence performance of the SpectraMax . Ferguson, Ph. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. 0 out of 5 $ 17,999 $ 16,995. 2. 0 . Flexible for 6-well to 384-well microplates and cuvettes. 3. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. ® reader to the computer. SpectraMax® M5. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). 1. Both readers feature an 8-channel read head for fast reads and. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. 6-384. This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Spectramax Multi-Mode Microplate Reader Performance Specifications. 7°C and the bottom to 36. Make sure the microplate is flat against.